Image-Pro Plus

Image-Pro Plus

Used by thousands of researchers worldwide, Image-Pro Plus image analysis software makes it easy to acquire images, count, measure and classify objects, and automate your work.

Image-Pro Plus software offers automated microscope control, image capture, measurement, count/size and macro development tools.

Capture with Ease

Accurate image analysis begins with acquisition. Utilize the full precision of your capture equipment with easy-to-use capture tools. Image-Pro Plus supports a wide range of digital cameras, image capture cards, and other devices.

Automated Microscope Control: Control complex microscopy equipment in a simple, repeatable manner for reproducible results. Automatically control your microscope stage in any X, Y, or Z direction and eliminate the need to manually adjust microscope settings.

Time Lapse Acquisition: Investigate changes in live specimens over time by acquiring images at predefined intervals. Play your time-lapse images as a movie to view movement and other activities.

Live Analysis & Measurements: Analyze samples live, without the need to acquire. The Workspace Preview feature makes it easy to count objects, apply measurements and interact with online images.

Process & Enhance

Use sophisticated image processing and enhancement tools to uncover details within your images.Image Alignment
Automatically align either individual or sequence images.

Imaging Tiling
Tile together a series of images or align sequence images to see a full representation of your research sample.

Extended Depth of Field
Create a focused image from a series of unfocused images with Extended Depth of Field.

Filter and Enhance
Choose from a variety of enhancement and edge filters to filter noise and improve image detail.

• Work with Filter dialogs that include descriptions and interactive preview windows
• Use Enhancement filters that include Low-Pass, Hi-Pass, Gaussian, Hi-Gaussian, Sharpen, Flatten, Median,
• Rank, Local Equalization, and De-speckle in user-definable kernel sizes
• Outline objects with various Edge Filter methods including Sobel, Roberts, Laplace, Variance, Phase, Horizontal, and Vertical
• Process objects with Morphological filters including Erode, Dilate, Open, Close, Top Hat, Well, Branch/End,
• Watershed, Thinning, Pruning, Distance, and Reduce in user-definable kernel sizes
• Work with Large Spectral Filters: Fast-acting, large kernel size (up to 4000 x 4000) filters that include Low-Pass, Hi-Pass, Band-Pass, and Edge filters
• Apply filters to individual frames or to the entire sequence
• Customize kernel files for morphological and convolution filters

Morphology
Use a wide range of morphological filters to precisely segment structures and prepare images for automatic measurement.

Background Subtraction & Correction
Eliminate background noise and correct uneven backgrounds in your images for improved analysis.

Original image

Background subtracted

New image

Merge Color Channels
Use the Color Composite feature to merge multiple fluorescent images acquired as monochrome single wavelengths into a color composite image. 

Co-localization
Detect co-localization in biological specimens and graphically display the association between the two sets of data in a scatter plot
Detect co-localization in fluorescent images. Mouse fibroblast cells – microtubules stained with FITC, mitochondria stained with Texas Red, nuclei stained with DAPI.

Pseudo-Color
Use pseudo-color to highlight features of interest in a grayscale image. Use colors to visually amplify specific intensities which are normally difficult to distinguish from their surroundings.

Fast Fourier Transforms
Perform forward and inverse Fast Fourier Transforms (FFT) to eliminate artifacts.

• Edit the spectrum directly including Low-Pass, High-Pass, un-sharpen, spike cut, and spike boost
• Save and reload data

Measure & Classify

Count and Classify Objects
Count and Characterize objects using over 50 manual and automatic measurement tools. Tag objects of interest and easily sort by size or other measurement parameters.

• Measure lengths, roundness, aspects, angles, areas, perimeters, and holes
• Set ranges for measurements to filter unwanted objects
• Group objects in classes based on measurements
• Resolve clustered objects with Watershed, Auto-Split, and Cluster analysis tools
• Use colors to separate and measure objects in color images
• Export measurements to statistical and spreadsheet package via DDE
• Display measurements as histograms & scattergrams

Threshold & Segmentation Tools
Use threshold and segmentation tools to extract objects of interest and distinguish them from other objects or from the background image.

Original image

Threshold values are set

Image after setting a threshold

Mask image based on threshold values

Threshold Color Images
Easily select objects by color. Use multiple classifications to segment and compare values of several different color groups.
Threshold and measure the percent area of objects.

Calibration Tools
Use the Image-Pro Plus calibration tools to redefine the scale of an image to express values in other units of measure. Learn more about Image-Pro Plus calibrations in the Spatial Calibration Product Note:

• Calibration wizard helps you to calibrate an active image and create reference and system calibrations
• Use X and Y scale bars as a nondestructive overlay
• Work with intensity or spatial measurements
• Create and display spatial calibration markers
• Create and display system calibrations
• Use pre-defined spatial calibration units
• Save and recall all calibrations
• Calculate derived calibrations automatically
• Store information about your microscope in lens information files

Metrology ToolsMeasure features such as best-fit line, arc, and circle.

Manual Measurements
Measure the lengths, areas, perimeters, and angles of objects in your image.

Calculate Thickness
Determine the thickness of objects by calculating the mean, minimum and maximum thickness between lines.

Trace Objects
Use the trace tool to highlight dark features on a primarily light background, such as fingerprint ridges. These features can be drawn manually or automatically. The automatic tracing tool works by tracing the skeleton of dark features.

Colocalization of Probes
The colocalization tools in Image-Pro Plus provide a visual representation as to the location and degree of overlap between two wavelengths.

Original fluorescence image.

Area of interest (AOI) within the scatterplot selects the colocalized areas (orange & yellow).

Mask shows overlapping regions of red and green.

Composite image showing FITC-labeled fibroblasts (green), Texas Red-labeled mitochondria (red).

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